1Directorate of Research, Translational Research for Veterinary Biologicals, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 051, Tamil Nadu
2Translational Research for Veterinary Biologicals, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 051, Tamil Nadu
Department of Animal Genetics and Breeding, Madras Veterinary College, Chennai-600 007, Tamil Nadu
*E-mail address: seevaganmm@gmail.com
Online published on 9 October, 2017.
Genetic variation in a part of growth hormone (GH) gene was studied in Vembur and Kilakarsal breeds of sheep. Two regions of GH gene viz., 214 bp fragment (includes part of intron 3, exon 4 and part of intron 4) and 365 bp fragment (includes part of intron 4, exon 5 and part of 3'UTR) were studied. DNAsequencing of GH gene revealed absence of SNPs in exons 4 and 5. It showed a T→C transition in Vembur breed of sheep in the 3 ’untranslated region of growth hormone gene. Genotyping of T→C transition mutation (T1965C) was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique using EcoP15I endonuclease and found to be polymorphic in Vembur sheep. Further studies are required to investigate the relationship between GH gene polymorphisms and the performance traits in these breeds.
, Growth hormone gene, Kilakarsal, Sheep, Vembur
