College of Biotechnology
2ICAR-Indian Veterinary Research Institute, Izatnagar-243 122, Uttar Pradesh
1Department of Veterinary Microbiology, UP Pandit Deen Dayal Upadhyaya Pashu-Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura-281 001, Uttar Pradesh
3Animal Health Division, ICAR-Central Institute for Research on Goats, Makhdoom-281 122, Uttar Pradesh
Sero-detection of brucella antibodies was done in 300 serum samples randomly collected from aborted goats by the classical serological tests like rose bengal plate test (RBPT), serum tube agglutination test (SAT) and rOMP31-based indirect-Enzyme-Linked Immunosorbent Assay (i-ELISA) and dot-ELISA. The seroprevalence for brucellosis was 22.66, 11.33, 24.33 and 17.33% with RBPT, SAT, i-ELISA and dot-ELISA, respectively. The sensitivity and specificity of different tests varied from 56.25 to 87.50% and from 75.00 to 90.00%, respectively. It was found that rOmp31-based ELISA is the most suitable test with 87.5% sensitivity and 90.0% specificity for detection of brucellosis in goats.
Brucellosis, Enzyme-linked immunosorbent assay, Goat, Rose bengal plate test, Serum tube agglutination test
