Isolation and identification of Lactate-Producing and utilizing bacteria from the rumen of najdi goats

A study was conducted during July, 2016 to September, 2017 on six Najdi goats. They were adapted to diet consisting of 60% concentrate for 3 weeks. Rumen fluid was collected and diluted to 10⁻⁶, 10⁻⁷, 10⁻⁸ and 10⁻⁹ concentrations. Lactate-producing and utilizing bacteria were isolated from the rumen fluid and identified by cultural, morphological and Gram's stain characteristics. The bacterial isolates were further confirmed by 16S rRNA gene PCR, sequence analysis and phylogenetic tree analysis by using MEGA5.2 software. Results indicated that the isolated lactateproducing bacteria strains (GP05, GP10) were 96% similar to Lactobacillus Fermentum E4 with Gene bank accession number MF179548.1 and 97% similar to Lactobacillus Fermentum KLDS 1.0733 with Gene bank accession number EU626018.1, respectively. Lactate-utilizing bacterial strains (GU1, GU2) showed 98% homogeneity to Megasphaera elsdenii JL1 with Gene bank accession number KX130893.1 and 99% homogeneity to Megasphaera elsdenii SA2 with Gene bank accession number KX130890.1, respectively. The bacterial isolates obtained from the rumen of Najdi goats can be used as probiotic for manipulation of gut microbial activity in animal nutrition.