Sequence analysis of ovine leptin and ghrelin genes in subfertile malpura ewes of Rajasthan

In the present study, leptin (exon 2 and exon 3) and ghrelin (exon 3 and exon 4) gene sequences were analysed for the presence of single nucleotide polymorphism and its association with subfertile condition in Malpura sheep. Leptin gene sequences revealed 11 non-synonymous (missense) and 8 synonymous mutations. Some of these missense mutations are novel to ovine leptin gene and not were reported earlier. Sixteen SNPs were observed in ghrelin gene sequences. Ghrelin allele A was not reported in true cyclic Malpura ewes which warrants further study.