1Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya, Evam Go-Anusandhan Sansthan, Mathura-281 001, Uttar Pradesh
College of Biotechnology, Mathura-281 001, Uttar Pradesh
*E-mail address: drvijaypandey@gmail.com
Online published on 23 September, 2021.
The study was conducted to extract and characterise cationic proteins from goat urine by acid urea polyacrylamide gel electrophoresis (AU-PAGE). The AU-PAGE gel of cationic fraction of goat urine showed 5 bands indicating presence of 5 cationic proteins in the urine. Moreover, fractions containing cationic proteins showed 23 mm and 26 mm of zones of inhibition in radial diffusion assay (RDA) against Staphylococcus aureus and Escherichia coli, respectively as compared to other fractions containing neutral/anionic proteins/peptides. The minimal inhibitory concentration (MIC) values for the same cationic urinary fractions were 0.0199 μg/μl and 0.039 μg/μl against S. aureus and E. coli respectively. Thus, it can be concluded that cationic fractions of goat urine contains cationic proteins/peptides which exhibit antimicrobial activity against test bacterial strains. However, further studies are required for scientific validation of these findings and establishing the antimicrobial activity of goat urine.
Acid urea polyacrylamide gel electrophoresis, Cationic proteins, Goat, Urine
