1Arid Region Campus, ICAR-Central Sheep and Wool Research Institute, Bikaner-334 001, Rajasthan
College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334 001, Rajasthan
*E-mail address: dr.harvinder.vet@gmail.com
Online published on 15 February, 2023.
A study was conducted to identify the polymorphism of keratin intermediate filament type-I (KIF-I) gene and its association with wool quality traits in Magra sheep. Blood samples and greasy fleece yield data of 162 adult males and females of Magra sheep (2 to 4 yr old) were collected. Following isolation of genomic DNA, genotyping of KIF-I gene was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The PCR-RFLP of KIF-I gene at locus KRT1.2 generated three genotype viz., AA, AB and BB with genotype frequencies of 0.49, 0.43 and 0.08, respectively. The frequencies for A and B allele were 0.71 and 0.29, respectively. Hardy-Weinberg equilibrium indicated that the population was in genetic equilibrium with respect to KIF-I gene. In conclusion, greater frequencies of allele A and genotype AA and AB are suggestive of its importance in quality of wool traits in Magra sheep.
Keratin intermediate filament gene, Magra sheep, Polymorphism, Wool traits
