Studies on extraction and purification of fibrolytic liquid enzymes and evaluating their effect on In vitro digestibility of cotton stalk in goats

In the present study, paddy straw-based fibre degrading liquid enzymes were extracted, purified, quantified and evaluated to assess in vitro digestibility of cotton stalks in goats. Cotton stalks were treated with liquid fibrolytic enzymes solution as T₁ : enzyme solution @ 250 ml/kg of cotton stalks, T₂ : water @250ml/kg of cotton stalks, T³ : enzyme solution @ 500 ml /kg of cotton stalks and T₄ : water @500ml/kg of cotton stalks. Treated cotton stalks were soaked for 4, 6 and 8 h before incubation. The results revealed that in vitro dry matter digestibility (IVDMD%) of cotton stalk was significantly higher (P<0.01) in T₁ for 6 (66.04±0.88) and 8 h(63.62±0.92) soaking period and in T₃ for 6 h(67.18±2.05) as compared to water treated (T₂ and T₄ ) cotton stalks. Significant (P<0.01) improvement was observed in in vitro neutral detergent fibre digestibility (IVNDFD%) in T₁ for 6 h (78.74±1.04) and in T₃ for 6 (80.46±1.90) and 8 h (74.80±1.65) as compared to other groups. in vitro acid detergent fibre digestibility (IVADFD%) was significantly higher (P<0.01) in T₁ for 6 (65.81±1.04) and 8 h (64.76±1.52) and in T₃ for 6 h (66.35±1.79).It was concluded that IVDMD, IVNDFD and IVADFD increased by liquid fibrolytic enzyme treatment of cotton stalks; however, enzyme treatment of cotton stalk @ 500 ml/kg and soaked for 6 h proved to be superior to other groups.