1Department of Veterinary Clinical Complex, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj-224 229, Uttar Pradesh
2Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj-224 229, Uttar Pradesh
Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj-224 229, Uttar Pradesh
*E-mail address: saurabh4208@gmail.com
Online published on 12 March, 2025.
Thirty ejaculates from five Barbari bucks (2 to 3 yr old) were collected to study the effect of supplementing ethanolic extract of Moringa oleifera leaves in extender on post-thaw motility of spermatozoa. Semen samples were diluted with tris-citric acid-fructose diluent to maintain sperm concentrations of 100 to 120 million per dose. Diluted semen was divided into three groups: control (C), T1 and T2 containing 0, 2 and 5% ethanolic extract of M. oleifera leaves, respectively. The diluents supplemented with 2.0 (T1) and 5.0% (T2) ethanolic extract of M. oleifera leaves had significantly (P<0.01) increased post-thaw sperm motility (55.33±0.49 and 62.17±0.65%), livability (76.17±0.65 and 79.33±0.67%) and HOS reactive sperm (36.50±1.93 and 39.00±1.95%) and reduced sperm abnormalities (12.17±0.8 and 10.33±0.9%) compared to the control (48.67±0.49, 74.50±0.76, 34.33±1.36 and 13.00±1.10%, respectively). Seminal plasma enzyme activities in post-thaw semen were also significantly (P<0.01) lower in T1 and T2 than control. Thus, it was inferred that supplementation of ethanolic extracts of M. oleifera leaves at 5% in the diluent improved the preservation of buck semen.
Barbari buck, Cryopreservation, Moringa oleifera leaf extract, Post-thaw motility, Semen
