Department of Gynaecology, Obstetrics and A.I. College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-22.
A total of 36 pooled ejaculates collected from four adult crossbred (Beetal x Assam Local) goats were utilized for the study. Seminal ejaculates were divided equally and extended in Tris-egg yolk-citricacid-glycerol extender containing either fructose, sucrose or lactose and frozen in liquid nitrogen using French medium straw after allowing glycerol equilibration period of either 1½, 1 or ½ hour before freezing. The mean per cent sperm motility and intact sperm acrosome after freezing was found to be significantly (P<0.05) higher in Tris extender containing fructose than in that containing sucrose or lactose in all the three glycerol equilibration periods used.
Sperm motility, acrosome, semen freezing, tris extender, fructose, crossbred goat