aDepartment of Biochemistry and Bioprocess Technology, S.H.I, of Ag., Tech. and Sci., Allahabad, U.P., India e-mail: depmala.kftiyar@gmail.com
bDepartment of Biochemistry and Bioprocess Technology, S.H.I, of Ag., Tech. and Sci., Allahabad, U.P., India e-mail: alokmilton@yahoo.com
cDepartment of Botany, Udai Pratap College, Varanasi, U.P., India e-mail: bhartidolly@gmail.com
1Corresponding author
Online published on 14 October, 2011.
Diatoms (Bacillariophyceae) are microscopic unicellular algae found in every aquatic habitat and contribute a large percentage of global carbon budgets through photosynthesis. A study was conducted to assess the effect of phosphate concentration on diatom growth. The freshwater diatoms were cultured in modified SAG media supplemented with different concentration (0.01 mM to 0.07 mM) of K2HPO4 as phosphate source and incubated at 20.0 ± 2.0°C and 4000 ± 10 lux (=lumem/m2) cool white bulb with 16 h of light and 8 h of darkness. The external supplement of K2HPO4 caused the phosphorus availability to be key factor in cell-division rate with increasing concentration of phosphate in medium. In this present study, it was found that Growth responses of diatoms showed gradual increase in the growth at 0.01, 0.02, 0.03, 0.04, 0.05 mM and gets maximum at 0.05 mM concentration of K2HPO4 whereas diatom growth was declined when concentration increases more than 0.05mM.
Diatom, phosphate SAG media
