Reduction of Phenolics and Endophytic Fungus in the Nodal and Internodal Explants of Jatropha Curcas L.

Jatropha curcus is being planted for the production of oil which in being used as biodisel. Plants generated from seeds are not superior. Therefore, Planting material regenerated through micropropagation is getting more importance. During tissue culture of Jatropha exudation of Phenolics and endophytic fungal growth cause great hindrance in the multiple shoot regeneration. In the present work attempt has been made to reduce, phenolics exudation and reduction in the growth of endophytic fungus. Three different antioxidant at different concentration have been used for the reduction of phenolics exudation where the concentration at 50mg/l each of ascorbic acid and citric acid have been found more suitable, which reduced exudation of phenolics to the greater extents. Here, 90% explants revealed inhibition of the exudation of phenolics. Among the fungicides used Bavistin at 1% concentration and 3 hours of duration of treatment was found more suitable than the rest of the fungicides either at the same concentration or at different concentrations. Fungicides at higher concentrations and even at 2 hours duration of treatment damage the explants severely which was perhaps due to toxic effects of the fungicides.