A Novel P 53 Target Gene Identified

Relation between cancer and p 53 has been reported in various studies since 1980s. Owing to the critical role of p 53 in controlling cancer, researchers have been working hard to identify and target p 53 genes with the view of designing drugs to suppress tumour growth and yet restore the protein function. In this study we report as a new p 53 target gene. A primer has been designed for LOC340109 and its expression was studied on p 53 and U20S-ADR cell lines. U20S-ADR cells are human osteosarcoma cells bearing wild p 53 treated with chemotherapy drug, Adriamycin. RT PCR was conducted and the products were then analysed on 1% agarose gel. LOC340109 over expressed in p 53 cells. LOC340109 expressed equally in all the U20S-ADR cells suggesting that LOC340109 is a p 53 target gene but has no effect by Adriamycin treatment. Hereby, form our preliminary identification study, we report LOC340109 as a new p 53 target gene.