siRNA Kockdown of TrpRS Induces Apoptosis, Inhibits Cell Proliferation and Arrests Cell Growth in Human Cervical Cancer Cells

TrpRS or Tyrptophanyl-tRNA synthetase is among the first proteins that appeared in evolution. The purpose of the present study is to evaluate the effect of silencing of TrpRS gene by RNA interference in human cervical cancer cells. Small interfering RNA (siRNA) was designed to knock down the expression of TrpRS gene. The effect of knockdown was studied by real time PCR and western blot. The effect of TrpRS silencing on cell proliferation was studied by CCK8 kit. Effect of silencing on apoptosis and cell cycle was studied by FACS. Real time PCR was performed to study the expression of BAX as well as the behavioral pattern of TrpRS in the cell cycle. The results revealed that siRNA knock down of TrpRS caused a gradual decline in the proliferation of cervix cancer cells, induced apoptosis, increased the expression of BAX gene, caused accumulation of cells in the G1 phase of cell cycle, also inhibited cell transition from G1 to S phase, in addition to inhibiting the expression of cyclin E & cdk2. These results suggest that, siRNA knock down of TrpRS gene on human cervix cancer cells may be subjected to rigorous study with a view to develop as gene therapy in patients with cervical cancer.