aDepartment of Environmental Science, VBS Purvanchal University, Jaunpur, Uttar Pradesh, India. E-mail: sudhirk7upadhyay@yahoo.co.in
bDepartment of Zoology, B.H.U., Varanasi, Uttar Pradesh, India. E-mail: sanjeevjnp@gmail.com
cDepartment of Environmental Science, B. B. Ambedker (Central) University, Lucknow, U.P., India. E-mail: dpsingh_lko@yahoo.com
1Corresponding author
Online published on 7 August, 2013.
Free living rhizospheric bacteria from wheat rhizosphere have capability to produced plant growth promoting attributes under saline condition. The electrical conductivity (ECe) of saline soil samples from four different districts viz. Varanasi, Mau, Ballia and Ghazipur of Uttar Pradesh-India was 10.47 to 5.69 dSm−1 and maximum bacterial population (6.9 X 106 cfu g−1 soil) was found on NA media as compared to rest of the media like King's B, Jensen's N free, TSA and SEA. About 33% of bacterial isolates were survive up to more then 8% NaCl (w/v) and only 19% showed PGP attributes at higher NaCl concentration. Ten identified PGPRs were used in the present study for their osmotolerence mechanism. Proline content was increased with NaCl stress and maximum production was recorded with Isolate SU8-Bacillus aquimarisviz. 2.73 and 11.95 g mg−1 protein at 0% and 10% NaCl (w/v) respectively. The tendency RS and TSS production in rhizobacterial isolates were reverse proportional to the salt (NaCl) concentration. Bacillus and bacillus derived genera were dominant under high saline condition along with PGP attributes, which could be mitigate salinity levels and improve agriculture crops under saline condition.
Osmolytes, PGPR, Proline, Reducing Sugars, Total soluble sugars, Rhizobacteria
