Identification, isolation and estimation of flavonoids and effect of growth regulators and salts on flavonoids in Aegle marmelos and Moringa oleifera in vitro

Unorganized tissues of medicinally useful plants Aegle marmelos and Moringa oleifera were established on MS medium supplemented with 1mg/L BAP+2mg/L 2,4-D and 1.5mg/L BAP +1.5mg/L 2,4-D respectively (standardized MS medium). Parts of established tissues were transferred to standardized (Sd) MS medium fed with various concentrations(1, 3, 5 mg/L) of growth hormones (IAA, NAA) and (10,20,30mg/L) salts (NaCl, KCl) separately. Tissues at the maximum GI (in all samples) were harvested, dried, powdered and analyzed for estimation of flavonoids. Maximum amount of flavonoids was calculated in callus fed with2mg/LIAA, NAAand10mg/LNaClandKCl in A. marmelos as well as M. oleifera.