Investigation of growth factors effect on in vitro culturing of Solanum melongena

Brinjal (Solanum melongena) is the second most important vegetable crop grown after potato in Asian countries and in various tropical and temperate parts of the world. It also plays a vital role in the National economy as a cash crop. Its tissues possessing a high morphogenetic potential that is useful for developmental studies as well as for establishing biotechnological approaches to produce improved varieties, such as transgenic plants. Here, stem and leaf explants were inoculated on MS medium with different hormones and different ratio for optimization of in vitro regeneration. Callus was initiated within a period of 10–12 days of culture and a mass of callus was formed in vivo and in vitro explants. In BAP: NAA 2.0mg/L:0.5mg/L, the callus induction started from the 12th day of inoculation. After 14 days, white hairy growth appears from light greenish callus which indicate the formation of root like structures. Callus induction was obtained from another hormone combination 2mg/L:0.5mg/L NAA: BAP. The Kinetin (2 to 3 mg/l) and BAP(2 to 3 mg/l) alone used for multiple shoot initiation. The shoot was initiated within a period of 45 days of culture from nodal explants. Even we have been used above 3 mg/L Concentration of NAA and IBA, Concentration of 2–3 mg/LNAA and IBA have been showed the optimal shoot initiation.