aBioscience and Biotechnology, Faculty of Engineering and Technology, University of Gezira, Sudan
bFaculty of Agriculture Abunaama, University of Sinar, Sudan
cNational Cancer Institute, University of Gezira, Sudan
dFaculty of Medical Laboratory, University of Gezira, Sudan
eNational Institute for Promotion of Horticultural Exports (NIPHE), University of Gezira, Sudan
1Corresponding author
Online published on 20 April, 2016.
Lectin was extracted from locally collected Anasazi beans (Phaseolous vulgaris), purified, precipitated by Ammonium sulphate and dialyzed against NaCl (0,145 M). Protein of the extracted lectin was estimated by the Lowery method.(1951). As sources of leukemic cells, freshly aspirated peripheral blood samples were collected from three previously diagnosed leukemic patients in Wad Medani Hospital. Two of the patients had acute lymphoblastic leukemia (ALL) and the third one had acute myelioblastic leukemia (AML). Density-gradient centrifugation was used for isolation of blast cells over 80%. Isolated lymphocytes were treated with Anazasi lectin at a concentration of 0.9 mg/ml. Vencristine was used as positive control at a concentration of 0.5/ml. Then, cell count for viable cells was done to determine anti-proliferation activity of lectin using Trypan Blue Exclusion Assay, after incubation periods of 3, 24 and 96 hrs of leukemic cells culturing. In this study, methods used for separation and culturing of leukemic cells were found efficient with mortality rate of less than 0.01% after 96 hrs for the leukemic cells used as control. Anasazi lectin exhibited In-vitro selective cytotoxic effect against leukemic cells isolated from relapsed patients with (AML) and (ALL). Difference among treatments and the control were found to be highly significant (P=). Lectin gave comparable results with vencristine at 3hrs, 24 hrs and 96 hrs of in-vitro preservation. Regarding effects of lectin and vencristine on leukemic cells results showed highly significant differences (p=) among blood samples (patients) with lectin gave 70.1, 92.85 and 91.25 for patient 1, patient 2 and patient 3, respectively; whereas, vencristine gave mortality rates of 70.8, 97.1 and 97.2 for patient 1, patient 2 and patient 3, respectively. Results also showed high progress in mortality of leukemic cells with time for lectin and vencristine. Results pointed lectin of Anasazi bean as an alternative safe anti-leukemic product.
Lectin, Agrobacterium tumefaciens, Leukemia, Vencristine, Relapsed patients, Myelioblastic, Lymphoblastic
