1FIST-DST Sponsored P. G. Department of Botany, Sri Murli Manohar Town P.G. College, Ballia, India
2Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, India
*Corresponding author
Online published on 21 May, 2018.
In Vitro study of antagonism was performed by dual culture method between the test pathogen, Macrophomina phaseolina and twelve strains of six Trichoderma species. Maximum inhibition of radial colony growth of M. phaseolina was observed due to T. harzianum IVRI (88.8%). The least effective antagonist for inhibition of radial colony growth of the pathogen was recorded due to T. virens 3067 (14.2%). Based on the results obtained for the colony interaction between different strains of Trichoderma species and the test pathogen, five potent strains of Trichoderma species were selected for further in vitro study. These were, T. harzianum IVRI, T. harzianum BHU, T. viride 1, T. pseudokoningii NBRI and T. virens BHU. The effect of different concentrations of culture filtrates of the different strains of Trichoderma species on per cent inhibition of radial growth of M. phaseolina was performed. Maximum inhibition of radial growth of M. phaseolina was observed in case of T. harzianum IVRI (42.2%), while minimum inhibition of radial growth was observed by T. virens BHU (31.2%) at 40% concentration.
In vitro Screening, Culture filtrate, , Trichoderma Macrophomina phaseolina, Antagonism
