Quantitative analysis of fatty acids composition and residual amount of ddts of Mystus gulio-A brackish fish

This study deals with the quantitative analysis of the fatty acid profile and the level of pesticides of fish lipid obtained from Mystus gulio. Lipid was extracted from Mystus gulio by ethylacetate, saponified and converted to methyl ester by using BF-MeOH in esterification process. The fish fillet was found to contain 18.22 mg/g of lipid. The TLC analysis revealed that the separation and identification of fatty acids whereas GLC analysis revealed the quantification of fatty acids. Among all the fatty acids oleic acid was predominant (30.29%). The amount of monounsaturated fatty acid (MUFA) was higher (34.08%) compared to the polyunsaturated fatty acids (PUFA) whose value was found to 14.42%. The fish fillet was found to contain ω-3 fatty acid (5.46%) and ω-6 fatty acid (7.35%). A very small amount of DDTs was found by GC-ECD in the fish fillet. The ratio of 4, 4 ΄-DDT/∑DDT, 4, 4-DDT/DDE and (DDD+DDE)/∑DDT, was 0.109, 0.176 and 0.745 respectively, indicates past input of DDT and also long term biotransformation of DDT to DDD and DDE.