Determination of amino acid contents in Achyranthes aspera, Cissus quadrangularis and Moringa oleifera

Plants are excellent source for the discovery of new products of medicinal value for drug development. Reliable methods of standardization are needed for the analysis of crude plant products. Chromatography is widely used in search for biologically significant molecules such as amino acids. The amino acids are the basic units of proteins and play a vital role in the metabolism of secondary metabolites. Taking into consideration the above facts the qualitative estimation of amino acids from the seeds of Achyranthes aspera (Apamarg) and stem of Cissus quadrangularis (Hadjor) and leaves of Moringa oleifera (Sahjan) have been carried out by paper chromatography. Two mobile phases were selected after employing various mobile phases for the detection of amino acids by paper chromatography technique. The results were compared with the standard amino acids in both mobile phases. Twelve amino acids in Achyranthes, fifteen amino acids in Cissus and Sixteen amino acids in Moringa were detected by using two different mobile phases.