Microbial degradation of 4-chlorobenzoic acid by Pseudomonas putida strain ps-i

Pseudomonas putida strain PS-I, a 4-chlorobenzoic acid degrading bacteria was isolated from soil. This bacterium could degrade 13 mM of 4-chlorobenzoic acid rapidly with stiochiometric formation of chloride ion. Metabolites such as 4-chlorocatechol and cis, cis-muconic acid could detect in the grown medium. Further, ring cleavage enzyme catechol 1, 2-dioxygenase detected in crude cell extract. Thus, the biodegradation pathway of 4-chlorobenzoic acid by this bacterium was proposed involve the 4chlorocatechol as intermediate compound and further this converted into cis, cis-muconic acid by the process ortho ring cleavage pathway.