Purification and characterization of catechol 1, 2-dioxygenase from Pseudomonas putida Strain PS-I

Pseudomonas putida Strain PS-I utilized 4-chlorobenzoic acid as a sole source of carbon and energy, degrading it through the ortho ring cleavage pathway. They synthesized the enzyme catechol 1, 2-dioxygenase which is unique in its properties to transform chlorocatechol of 4-chlorobenzoic acid in to chloro cis-cis muconic acid, while catechol 1, 2-dioxygenase is only efficient degrader of catechol in to cis-cis muconic acid. The enzyme was purified to homogeneity by ion exchange and gel filtration chromatography from culture supernatant. The purified native had an estimated molecular mass 55 kDa and it is composed of two sub units each of 25 kDa determined by SDS-PAGE.