Screening of Supplementary Nitrogen Source for L-asparaginase Production

The demand for L-asparaginase will increase several fold in coming years due to its potential application as food processing aid in addition with clinical applications. It has been observed that eukaryote microorganisms like yeast and filamentous fungi have a potential for L-asparaginase production. In the present study L-proline was used as substrate and the independent effect of ammonium chloride, urea and sodium nitrate as supplementary nitrogen source on the production of extracellular L-asparaginase by Aspergillus terreus MTCC 1782 was investigated using 5-level Latin square design. The statistical reliability and significance of the variables was studied by performing Analysis of Variance (ANOVA) for experimental L-asparaginase activity using Data plot software. Dex Mean plot of Graphical ANOVA was used to evaluate the effect of supplementary nitrogen sources on L-asparaginase production by A. terreus MTCC 1782. The best supplementary nitrogen source (with maximum confidence level) was found for maximum L-asparaginase production by Aspergillus terreus MTCC 1782 using L-proline as substrate in submerged fermentation.