Department of Physics, Tripura University, Suryamaninagar-799 022 (Tripura)
*E-mail: sa_h153@hotmail.com
Online published on 5 August, 2015.
Optrodes are basically synonyms of fluorescence sensors. Sensing of pH and temperature has wide applications in analytical, biomedical and clinical chemistry. The present work reports the investigations on the properties of a cationic laser dye Acriflavine (Acf) in solution and in Layer-By-Layer self assembled films to explore its possible application as pH sensor and temperature sensor. It has been observed that 35 minute is required to complete the adsorption process of Acf on to LBL films. The fluorescence spectra of Acf in solution and in LBL films were pH sensitive over a wide pH range 2.5–13. Fluorescence intensity shows hysteresis during increase and decrease of pH in both solution and LBL films. Both UV-Vis absorption and fluorescence spectra were found to be temperature sensitive.
Optrodes, fluorescence sensors, chromophore, layer-by-layer