Resistance in mungbean against five variants of Mungbean yellow mosaic virus

Resistance in mungbean (Vigna radiata L.) genotypes against five distinct variants of Mungbean yellow mosaic virus (MYMV) viz. Pp1, Bg3, MbD, MoL and MbS was evaluated in separate insect-proof glasshouses through whitefly (Bemisia tabaci Genn.) transmission. Resistance in genotypes varied from variant to variant. The genotypes resistant under glasshouse conditions, were also resistant under field conditions. The mungbean genotypes MGG 443, ML 5, ML 337, ML 459, ML 513, ML 610, P 9271 and P 9272 were identified as the most promising resistant genotypes. Symptomless infection in a few genotypes of mungbean was identified by nucleic acid spot hybridization (NASH) using radiolabelled specific MYMV DNA probes. It was also found that the accumulation of viral DNA was less in resistant genotypes than in susceptible genotypes.