1Sugarcane Research Station, Kunraghat, Gorakhpur-273008, India.
2UMR 385, CIRAD-ENSAM-INRA Biologie et Genetique des Interactions en Recherche Agronomie pour le Devloppment, Campus International de Baillarguet, TA 41/K, 34398 Montpellier Cedex 5, France.
Abstracts of Research Papers Presented during the National Symposium of Indian Virological Society at Unit of Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi-110 012, October 14–1.
Seven sugarcane and sorghum leaf samples exhibiting mosaic symptoms and that originated from (Uttar Pradesh, Maharashtra and Tamil Nadu) were characterized through RT-PCR assays with specific designed primer for Sugarcane mosaic virus, SCMV (F4 and R3), Sugarcane Streak mosaic virus SCSMV (ST2 and P1) and SrMV (F3 and R3). SCMV was detected by RT-PCR in 3 sugarcane varieties. The expected 0.9 kbp SCMV fragment was amplified by RT-PCR with the SCMV F4 and SCMV R3 primers with mosaic samples from Maharashtra (cv.VSI-9-20 and CoM 9086) and Tamil Nadu (cv. Co740) However, ca 0.5 kbp fragment was amplified from six of the samples out of seven tested using primers SCSMV-ST2 and SCSMV-P1. RT-PCR reactions were negative with SrMV primers pairs in all the tested samples. Our results suggest widespread existence of SCMV in India in comparison to the SCSMV. Interestingly, mixed infection of SCMV and SCSMV was also detected in sugarcane mosaic infected leaf samples from Maharashtra and Tamil Nadu.
