Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, U.P.
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, U.P.
Abstracts of Research Papers Presented during the National Symposium of Indian Virological Society at Unit of Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi-110 012, October 14–1.
The VP2 gene of canine parvovirus was amplified by polymerase chain reaction and cloned in pTargeT mammalian expression vector. The recombinant plasmid containing VP2 gene in right orientation was selected on the basis of restriction enzyme analysis and further confirmed by sequencing. The recombinant plasmid pTargeT.cpvp2 was used to transfect CRFK cells and found to express VP2 protein as detected by immunoperoxidase test. It was used as DNA vaccine in dog by injecting as 100 mg DNA with and without adjuvant, and keeping vector alone and healthy control groups. The dogs injected with recombinant plasmid alone or with adjuvant ISA50 (10%v/v) were protected fully from the disease whereas dogs injected with vector alone and healthy control dogs died/suffered from the disease. The serum neutralizing antibody titre in vaccinated dogs was 1:256 and 1:512 while vector alone and healthy control group dogs did not show any SN antibody response. The present work has shown that the recombinant plasmid could be used as DNA vaccine against canine parvovirus infection.
