1Indian Agricultural Research Institute Regional Station, Agricultural College Estate, P.O. Shivajinagar, Pune-411005.
2Plant Virology Unit, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi-110012.
*Corresponding author E-mail: rajverma2006@rediffmail.com
During survey, commercial fields of bottle gourd (Lagenaria siceraria) plants exhibited severe mosaic, interveinal chlorosis, and leaf deformation that resulted in fern-leaf appearance and severe fruit distortion in approximately 70% of the plants. The virus isolate was identified as Zucchini yellow mosaic virus (ZYMV) by biological and serological techniques, electron microscopy and RT-PCR. Nucleotide sequencing of the cloned PCR product showed that it was 497 bp long. Sequence analysis indicated that it contained C-terminal part of coat protein region and 3’ untranslated region (UTR). The C-terminal coat protein region showed 100% sequence identity with most of the isolates of ZYMV while 3’UTR region shared sequence identity in the range of 86.8% to 99.5% with different isolates of ZYMV indicating that the virus infecting bottle gourd is an isolate of ZYMV.
Bottle gourd, ZYMV, insect transmission, coat protein, UTR
