1Institute of Agri-Biotechnology, University of Agricultural Sciences, Dharwad, India.
2Department of Genetics and Plant Breeding, University of Agricultural Sciences, Dharwad, India.
3ACSIP, University of Agricultural Sciences, Dharwad, India.
An efficient in vitro plant regeneration protocol through callus and direct mode using various explants was optimized for ICPL 87119 and ICP 8863. Large number of plants were regenerated and established in pots (SC0). Variations for several traits including for the incidence of SMD was noticed in SC1 generation (progeny-to-row). Typical symptoms of SMD were observed stating from seedling stage till the end of the life cycle. Severity of the symptoms varied across progenies at seedling and later stage. In general, progenies that exhibited complete susceptibility to complete immunity were noticed in SC1 generation, both within a row as across. The spectrum of variation of various traits including SMD incidence was almost same between ICPL 87119 and ICP 8863. Progenies with SMD resistance coupled with other desired variations (like earliness, plant type etc.) were also noticed. Selected progenies from SC1 generation forwarded to SC2 and the behavior of observed traits at SC1 were studied. In SC2 generation the spectrum of variation observed was much higher than SC1 generation. Variation for plant type was significantly high in SC2 generation. New phenotypes including the progenies that look like wild type pigeonpea were also observed. Segregation for SMD resistance coupled with plant type was noticed in a few SC2 lines. Details on symptoms and spectrum of variation in incidence of SMD, segregation and different traits across generations and lines will be discussed.
