Indian Journal of Virology

  • Year: 2006
  • Volume: 17
  • Issue: 2

S.56. Monitoring for B biotype in certain whitefly (Bemisia tabaci) populations collected from different locations in India

  • Author:
  • K.S. Shankarappa1, K.T. Rangaswamy1, N. Raghavendra1, D.S. Aswatha Narayana1, C.N. Lakshminarayana Reddy1, H.A. Prameela1, J. Colvin2, V. Muniyappa1
  • Total Page Count: 2
  • DOI:
  • Page Number: 127 to 128

1Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bangalore-560065.

2Natural Resources Institute, University of Greenwich, Chatham Maritime, Kent ME4 4TB, U.K.

Abstracts of the papers presented at the 16th Annual Convention and International Symposium of Indian Virological Society on “Management of Vector-Borne Viruses” at International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru-502324, Hyderabad, India, February 7–10, 2006.

Abstract

A survey was made to test certain Indian populations of whitefly Bemisia tabaci for the presence of B biotype. The study involved application of PCR technique using OpB 11 primer and B biotype specific SCAR primers (Forward 51 ACCC GTTG AATC CTAT AGAC-31 and Reverse 51-TGTT ATGC TTAC CCGG AAC-31) developed based on information on base sequence of B biotype specific 750 bp (~) RAPD fragment obtained with OpB 11 primer and observation of the Pumpkin silver leaf reaction. The results obtained by three methods were consistent and showed that the Indian populations of B. tabaci were composed of two genetic types. One corresponded to populations of B biotype with a distinct RAPD product 750 bp (~) and PCR product 548 bp with OpB 11 and B biotype specific SCAR primer respectively found only in some populations from southern Karnataka. The other showing a distinct product of 550 bp (~) with OpB 11 and non amplification of B biotype specific 548 bp product similar to Indigenous strain (UAS (B) stock culture) was found in Ludhiana, Mukthasar, Abohar, Bhantida, (Punjab), Parbhani, Aurangabad (Maharashtra), Coimbatore, Salem (Tamil Nadu), Hyderabad (Andra Pradesh), Trivandrum (Kerala) and New Delhi, as well as in some populations from Northern and Coastal Karnataka.