Indian Journal of Virology

  • Year: 2006
  • Volume: 17
  • Issue: 2

P.50. Studies on transient expression of coat protein (CP) gene of Papaya ring spot virus (PRSV) towards developing transgenic resistance in papaya

  • Author:
  • K.N. Chandrashekara, G. Ashok Kumar, K.B. Krishnamurthy, K. Jagadish, S. Rashmi, Akella Vani
  • Total Page Count: 1
  • DOI:
  • Page Number: 144 to 144

Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta, Bangalore.

Abstracts of the papers presented at the 16th Annual Convention and International Symposium of Indian Virological Society on “Management of Vector-Borne Viruses” at International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru-502324, Hyderabad, India, February 7–10, 2006.

Abstract

The viral disease caused by Papaya ring spot virus (PRSV) has become a serious threat to papaya cultivation worldwide. The coat protein (cp) gene from the local (Bangalore) isolate of the virus was cloned and sequenced. A plant expression cassette was developed and the plasmid construct was electroporated into the growing meristem of seedlings of papaya varieties Solo and CO-7. Studies on transient gene expression in detached leaves of papaya and tomato were carried out to validate the clone. The plasmid DNA (pAVCP-PRSV) was electroporated into leaf disks of papaya and tomato. Transient expression of PRSV-CP mRNA was studied at 1, 4, 8 and 16 hour intervals through RT-PCR followed by dot blot of the PCR product