1Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bangalore-560065.
2Agriculture College, V.C. Farm, Mandya, Karnataka.
Abstracts of the papers presented at the 16th Annual Convention and International Symposium of Indian Virological Society on “Management of Vector-Borne Viruses” at International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru-502324, Hyderabad, India, February 7–10, 2006.
The whitefly Bemisia tabaci is an efficient vector of geminiviruses worldwide. There has been an increasing incidence of geminiviruses on agricultural and horticultural crops in southern Karnataka after the introduction B biotype of B. tabaci. The importance of this biotype has promoted a great need for a rapid identification of geminiviruses in the vector. In this study, PCR technique was used to detect Jatropha mosaic virus (JMV) in individual adult whitefly of B. tabaci, B biotype using Deng primers, which amplified product of approximately 0.5kbp. The persistence of the virus in the viruliferous adult whitefly vector was monitored by PCR. The virus was detected in individual viruliferous adult B. tabaci till their death. However the intensity of the amplified product reduced gradually with the increase in the incubation period, which clearly indicated that the persistent circulative type of virus-vector relationship. This approach was also useful in detection of the virus in Cuscuta subilclusa a dodder routinely used for experimental transmission of JMV to various host species.
