Indian Journal of Virology

  • Year: 2006
  • Volume: 17
  • Issue: 2

P.05. Biological and molecular characterization of six isolates of Helicoverpa armigera Nucleopolyhedrovirus (HaNPV) from different geographic locations India

  • Author:
  • K. Sireesha1, G.V. Ranga Rao1, P. Arjuna Rao2, P. Lava kumar1
  • Total Page Count: 1
  • Page Number: 160 to 160

1International Crops Research Institute for the Semi-Arid Tropics, Patancheru-502324, Andhra Pradesh.

2Acharya N.G. Ranga agricultural University, Agricultural College, Bapatla, Andhra Pradesh.

Abstracts of the papers presented at the 16th Annual Convention and International Symposium of Indian Virological Society on “Management of Vector-Borne Viruses” at International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru-502324, Hyderabad, India, February 7–10, 2006.

Abstract

Comparative virulence of six isolates of HaNPV was assessed through concentration-mortality responses of second and third instar larvae of Helicoverpa armigera by diet surface contamination method. Concentration-mortality response against third instar on 9th day showed that the ICRISAT-HaNPV isolate was most promising with lower lethal concentration at 50% mortality (LC50) value of 1.5×103 POB/ml followed by GAU-HaNPV (1.9×103 POB/ml), PAU-HaNPV (2.26×103 POB/ml), TN-HaNPV (2.3×103 POB/ml), AK-HaNPV (3.1×103 POB/ml) and UASD-HaNPV (3.7×103 POB/ml). Observations with second instar also showed similar trend, whereas ICRISAT-HaNPV found superior with lower LC50 value of 3.25x102 POB/ml followed by GAU-HaNPV (3.43×102 POB/ml), PAU-HaNPV (5.28x102 POB/ml), TN-HaNPV (7.33×102 POB/ml), AK-HaNPV (8.18×102 POB/ml) and UASD-HaNPV (9.99×102 POB/ml). The six HaNPV isolates were purified and morpho-physical characters were compared in by electron microscopy and SDS-PAGE. Results of this study will be discussed.