Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh.
Abstracts of the papers presented at the 16th Annual Convention and International Symposium of Indian Virological Society on “Management of Vector-Borne Viruses” at International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru-502324, Hyderabad, India, February 7–10, 2006.
The rabies recombinant plasmid was cultured on large scale in 8 litre volume in a 10 litre bioreactor vessel with air flow rate 2 litre/min, agitation speed 180–200 rpm, temperature 37°C, pH 7.2. The cells were pelleted using Sorvall RC3C centrifuge with 6 x 500 ml centrifuge bottles. It was further processed for plasmid DNA isolation by alkaline lysis method. The supernatant obtained was applied to silica gel column for DNA isolation and purification. The plasmid DNA quality and concentration was found comparable with plasmid DNA isolated using commercially available kit. Further improvement in plasmid DNA isolation process is underway.
