1Department of Plant Pathology, University of Agricultural Sciences, Dharwad 580005.
2Indian Institute of Horticultural Research, Bangalore 560 089.
*Corresponding author; E-mail-byadgi_uas@rediffmail.com
Papaya ring spot virus (PRSV) producing mosaic, deformation of leaves and stunting on mechanically inoculated Curubita pepo L. ‘Goldy’ was isolated. Purified virus particles were flexuous rod in shape. Polyclonal antiserum for PRSV was produced by immunizing rabbit. Immunocapture RT-PCR amplified 550 bp coat protein gene. Enzyme-linked immunosorbent assay for skin of diseased papaya, fruit and stem was mild positive but was negative for flowers, latex, seeds and roots from infected plants. DIBA detected virus in infected plant samples. Tissue imprinting of PRSV infected squash leaves revealed more localization of virus in interveinal regions as compared to veins.
Papaya ring spot virus, polyclonal antiserum, inclusion bodies, immunocapture RT-PCR, DIBA, DAC-ELISA, tissue imprinting
