1Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122.
2Deptt. of Animal Science, M.J.P. Rohilkhand University, Bareilly 243006.
Recombinant plasmid containing fowl adenovirus 4 hexon gene designated as pCDNA3.1/V5-His-TOPO was digested with 17 restriction enzymes i.e. PstI, BamHI, EcoRI, HindIII, XmaI, XhoI, BglII, SpeI, SacI, SalI, DraI, AluI, AccI, XbaI, HhaI, KpnI and TaqI with their specific assay buffers. DNA fragments of expected size was observed and confirmed the right orientation of insert gene as analyzed on 0.7% agarose gel electrophoresis. It was found that enzymes BamHI, PstI, HindIII, SpeI, DraI had no site on insert DNA while EcoRI and XbaI had no site on vector as well as on insert DNA.
Fowl adenovirus 4, hexon gene, restriction enzyme
