1Plant Virology Lab, Institute of Himalayan Bioresource Technology, Palmapur-176061.
2Department of Mycology and Plant Pathology, Dr YS Parmar University of Horticulture and Forestry Nauni, Solan-173230.
*Corresponding author. E-mail: zaidi_aijaz@yahoo.com
Apple chlorotic leaf spot virus (ACLSV), a Trichovirus, was detected in 17 different cultivars of apple collected from different regions of Himachal Pradesh and in 4 rootstocks which are extensively used throughout India by direct antigen coating indirect ELISA. The sequence of an amplicon produced by RT-PCR using a degenerate primer confirmed the ELISA results for the cultivar Royal Delicious collected from the Kinnaur area. This study confirms the presence of ACLSV in India. The Kinnaur isolate falls under group A of ACLSV.
ACLSV, detection, RT-PCR, coat protein
