1R&D Division, Bangalore Genei, SSCL, Bangalore-560058, India
2University of Florida, Citrus Research and Education Center, Lake Alfred, FL, 33850, USA.
Indian Citrus tristeza virus (CTV) isolate B165 induces severe vein flecking in Coorg mandarin and seedling yellows on sour orange and grapefruit. The viral genomic RNA was sequenced and compared to published CTV genomes. The genome of the major component of B165 was 19247 nt in length with 12 ORFs and was organized identical to other CTV isolates. All sequenced CTV isolates were 2–55 nt longer than B165, except for VT which was 21 nt shorter. The 3´ untranslated region (UTR) had more than 97% identity but only 59–78% identity was observed in 5´UTR with others. B165 had nucleotide identity in the regions of ORF2 to 11 was 92–99% and 71–86% in the ORF 1 with all sequenced CTV isolates. B165 should be considered as a new genotype based on the sequence identity in the ORF1 with other reported CTV genotypes. Nucleotide sequence data are available in the GenBank database under Accession Number EU076703.
