P-15. DNA sequence analysis of Citrus yellow mosaic virus isolated from acid lime and pummelo
Abstract
Citrus yellow mosaic badna virus (CMBV), a member of the Genus badnavirus, is associated with the yellow mosaic disease observed in several citrus species in Southern India. CYMV, isolated from sweet orange has been reported to be having a circular double-stranded DNA genome whose size is about 7.5 kb. To investigate the relationship between the viruses associated with the disease in acid lime, pummelo and sweet orange, leaf samples collected from symptomatic acid lime and pummelo plants were used to extract total DNA using the method of Kobayashi et al, (1998). This was then used to generate amplification products measuring 3.2 kb and 4.3 kb employing synthetic oligonucleotide primers derived from the nucleotide sequence of sweet orange isolate of CYMV. The position of the two primers was such that the two products will have an overlap of … bp, and together would represent the entire CYMV genome. The expected amplification products were obtained and were cloned in … and completely sequenced. The sequence of the two isolates and their comparison with sweet orange isolate will be presented.
