Indian Journal of Virology

  • Year: 2008
  • Volume: 19
  • Issue: 1

P-61. Molecular evidence for natural occurrence of Bhendi yellow vein mosaic virus in Urena lobata from eastern India

  • Author:
  • Sanchalika Acharyya, Sujay Paul, Raju Ghosh, Subha Das, Paramita Palit, Arnab Das, Javid Iqbal Mir, Subrata Kumar Ghosh, Anirban Roy
  • DOI:
  • Page Number: to

Plant Virus Laboratory and Biotechnology Unit, Division of Crop Protection, Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata-700120, India.

Abstract

Urena lobata L. is popularly known as Aramina, Caeser weed or Congo jute and belongs to the family Malvaceae. The occurrence of yellow vein mosaic symptoms in endemic form throughout the eastern India and the association of a Begomovirus with this disease have already been reported but the identity of the causal virus is still obscure. To explore this, in the present study, association of the Begomovirus was confirmed by low stringency hybridization using a radiolabelled DNA-A probe of Mesta yellow vein mosaic virus (MeYVMV) (EF428256). PCR amplification using the sample DNA with the primer pairs designed to the coat protein of Bhendi yellow vein mosaic virus and universal DNA £] primers yielded the fragments of approximately 0.77 kb and 1.0 Kb respectively from the samples showing the symptoms but not from those without symptoms and thus confirmed the presence of DNA-A and a defectiveDNAfÒ with the symptomatic samples tested. These amplification products were then cloned and sequenced. The sequence analysis of coat protein gene (EU184017) showed that it shared high sequence identity both at nucleotide and amino acid level with different isolates of Bhendi yellow vein mosaic virus (BYVMV). While at nucleotide level, it shared highest identity (97.2%) with BYVMV variant 2 reported from Madurai, south India (AJ278860) followed by BYVMV barrackpore isolate (EF417918) reported from east India (97%), at amino acid level it showed highest identity (99.6%) with BYVMV Barrackpore isolate. The analysis of DNA £] (EU188922) showed that this molecule consisted of 1079 nucleotide and lack the N-terminal region of £] C1 ORF and thus appeared to be a defective DNA £]. This defective DNA £] shared highest identity (77.9%) with that reported to be associated with Barrackpore isolate of Bhendi yellow vein mosaic virus (EF417919). The result thus showed the molecular evidence for association of east Indian isolate of Bhendi yellow vein mosaic virus and a defective satellite DNA £] with the yellow vein disease of Urena lobata and thus highlighted that such plants act as a reservoir of Bhendi yellow vein mosaic virus posing a serious threat to the bhendi crops in eastern India.