Plant Virus Laboratory and Biotechnology Unit, Division of Crop Protection, Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata-700120, India.
Abstracts of the papers presented at the International Conference of Indian Virological Society on “Emerging and Re-emerging viral Diseases of the Tropics and Subtropics” at Indian Agricultural Research Institute, New Delhi, India, December 11–14, 2007.
Yellow vein disease of Bhendi, caused by a complex of Bhendi yellow vein mosaic virus (BYVMV) and a satellite DNA£] fz poses a serious threat, and very little information about the variability of the causal complex at different agro-ecological situations in India is available. Present investigation was conducted with BYVMV isolate obtained from eastern India to know its molecular status and phylogenetic relationship based on coat protein and satellite DNA£] sequences. The sequence of coat protein gene of the present isolate (EF417918) shared highest sequence identity, both at nucleotide and amino acid level, with a variant of the south Indian isolate (99.7% and 100% respectively, Accession no. AJ278861). Whereas sequence of present DNA£] molecule (EF417919) shared highest sequence identity with that of Pakistan (95.6%, Accession No. AJ316030). The result thus indicated that eventhough east Indian BYVMV appeared to be a related strain of southIndian one but the east Indian DNA£] fnmoleculefz, the other component of the etiological complex associated with the disease, is characterized to be different from that of south India. In the present investigation, multiple nuclear localization signals (NLSs) at the N-terminal region of coat protein and endoplasmic reticulum membrane retention signal in £]C1 protein of DNA£] were also determined. Present study not only reveals the novelty of this East Indian isolate in its phylogenetic relationship with the isolates from other parts of India and Pakistan but also helps to predict the probable functional aspects of the coat protein gene and the £] C1 protein.
