The present study was undertaken to evaluate and compare the efficacy of the diagnostic tests such as haemagglutination, haemagglutination inhibition tests, and dot-ELISA for Canine parvovirus (CPV). Ninety four faecal samples were collected from CPV suspected dogs in and around Puducherry. A total of 27 samples (28.7%) samples were found to be positive by haemagglutination test and subsequently confirmed by haemagglutination inhibition test. Totally 47 samples (50%) were detected positive by dot-ELISA. The occurrence of CPV infection in Puducherry was highest during the period from June to August and dogs below one year of age were predominantly affected. Antigenic characterization of 27 faecal samples using a panel of four monoclonal antibodies revealed 22 samples as CPV-2a and the remaining five as CPV-2b. Comparative evaluation of the diagnostic tests revealed higher sensitivity and lesser time requirement for dot-ELISA and hence can be used in the field for rapid diagnosis of CPV infection.
Canine parvovirus, diagnosis, antigenic typing
