Plant Virus Laboratory and Biotechnology Unit, Division of Crop Protection, Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata-700120.
*Corresponding author: Anirban Roy, (E-mail: anirbanroy75@yahoo.com)
Coat protein gene of an isolate of Bhendi yellow vein mosaic virus (BYVMV) and the associated DNA-beta satellite obtained from eastern India were cloned and nucleotide sequences were analyzed to know its molecular status and phylogenetic relationship. Multiple nuclear localization signals (NLSs) were found at the N-terminal region of coat protein and endoplasmic reticulum membrane retention signal in βC1 protein of beta satellite. The sequence of coat protein gene shared highest sequence identity, with that of the south Indian isolate (BYVMV-[Mad]:Var3, Accession no. AJ278861) and formed cluster with it, whereas the beta satellite molecule (BYVB-[IN:Bar:06]), characterized in the study, shared highest sequence identity and formed cluster with that recorded to be associated with another isolate of BYVMV on bhendi plants from Pakistan (BYVB-[PK:Bah:97]) (Accession No. AJ316030).
Bhendi yellow vein mosaic virus, DNA beta satellite, coat protein, cloning, polymerase chain reaction, phylogenetic analysis
