P-62. Molecular characterization and variability analysis of Apple scar skin viroid
Author:
Y. Walia1, Y. Kumar1, T. Rana1, V. Hallan1, R. Ram1, P.D. Thakur2, U. Sharma3, A. Handa2, A. A. Zaidi1
Total Page Count: 1
DOI:
Page Number: 43 to 43
1Plant Virology Lab, Institute of Himalayan Bioresource Technology, CSIR, Palampur-176 061, Himachal Pradesh, India.
2Department of Mycology and Plant Pathology, Dr. Y. S Parmar University, Nauni, Solan, India.
3KVK, Rohru at Shimla, India.
Abstract
Apple scar skin viroid (ASSVd) infection is a major limitation to apple fruit quality and causes huge economic losses. In surveys of apple orchards of Himachal Pradesh (H.P.), fruits were noticed which showed dappling symptoms. ASSVd was detected from them and molecularly characterized. Ten clones from three isolates were sequenced out of which seven new sequence variables of ASSVd were found. The clones showed significant sequence variability (94–100%) with each other. Variability was more common in the pathogenic domain of the viroid genome. Four of the clones were 330 nucleotides (nt) long and the other six had an additional nucleotide. Phylogenetic analysis showed close affinity of the present isolates with some Chinese and Korean isolates. The study reports seven new variables of ASSVd and also gives a first molecular evidence of a viroid infection (ASSVd) in apple from India.
