1Department of Plant Pathology, N.D. University of Agriculture and Technology, Kumarganj, Faizabad-224 229.
2Advanced Center of Plant Virology, Department of Plant Pathology, IARI, New Delhi-110 012, India.
Abstracts of the papers presented at the XVIII National Conference of Indian Virological Society at Post Graduate Institute of Medical Education and Research, Chandigarh, India, December 11–13, 2008.
Chilli plants showing severe mosaic mottling on foliage and bud necrosis symptoms, were collected from different location around Faizabad and the causal virus was purified. The purified virus sample reacted only with polyclonal antiserum raised against coat protein (cp) of Tobacco streak virus (TSV) isolate from India (TSV -SF) in direct antigen coated enzyme linked immunosorbent assay. The identity of the causal virus associated with chilli bud necrosis was further confirmed by reverse transcription polymerase chain reaction and sequenced analysis. The CP gene was amplified and sequenced. The CP gene was 717 nucleotides long and could encode a protein of 238 amino acids. Comparative aminoacid sequence analysis revealed that the virus infected chilli shared maximum identity both at nucleotide (98–99% and amino acids (98%) levels with the corresponding region of TSV isolates.
