P-69. Apple stem pitting virus diagnosis on apple in India
Author:
S. Dhir1, M. Tomar2, P.D. Thakur2, R. Ram1, V. Hallan1, A.A. Zaidi1
Total Page Count: 1
DOI:
Page Number: 45 to 45
1Plant virology Lab, Institute of Himalayan Bioresource Technology, CSIR, Palampur-176 061, HP, India.
2Department of Mycology and Plant Pathology, Dr. Y.S. Parmar University of Horticulture & Forestry, Nauni, Solan-173 230, India.
Abstract
Apple stem pitting foveavirus (ASPV) is a major virus of pome and stone fruits, and thus its reliable diagnostics is a crucial step in virus-free material production and prevention of ASPV spread by infected graft woods. Thirty samples from several apple varieties from various locations of Himachal Pradesh were analyzed for ASPV by ELISA (Bioreba, Switzerland) and RT-PCR. Positive result was obtained for 6 varieties namely Starking delicious (Royal delicious), Red fuji, Red chief, Silver spur, Red spur and Well spur. Total RNA was extracted from ELISA positive leaf and bark samples using RNA isolation kit (Qiagen, Germany). RT- PCR was performed using primer pair with target sequences in the fifth open reading frame of ASPV genome. An amplicon of ~370 bp was obtained which was cloned into pGEM-T easy vector (Promega, USA) and sequenced. A sequence of 366 bp was obtained (Accession no. FM863704). The sequence was related to ASPV CP gene and was 99% similar to the Chinese isolate (Accession No. ACD43643). Based on ELISA and sequence data, isolate was identified as Apple stem pitting virus. To the best of our knowledge, this is the first report of ASPV from India.
