Department of Microbiology & Animal Biotechnology, Nagpur Veterinary College, Nagpur, India.
Abstracts of the papers presented at the XVIII National Conference of Indian Virological Society at Post Graduate Institute of Medical Education and Research, Chandigarh, India, December 11–13, 2008.
The present investigation was carried out to study the prevalence of canine parvovirus in healthy as well as diarrhoeic dogs. In all 27 faecal samples from healthy as wellas diarrhoeic dogs aged ranged from 2 months to 1 year were collected. The fecal samples were suspended in PBS at a concentration of 10 per cent. For Canine Parvo Virus-2 (CPV-2) detection, the DNA was extracted using alkaline extraction method. For CPV-2 DNA detection VP2 gene based Polymerase chain reaction(PCR) was employed. Out of 27 faecal samples screened, 2 were found positive for CPV-2 by VP2 gene based PCR analysis. From the present study it is evident that the PCR is rapid test for dignosis of canine parvo virus infections in dogs. Department of Virology. PGIMER, Chandigarh.
