In the present study faecal samples were collected from seventy one bovine calves and one hundred seventy two ovine lambs below one month of age from various government farms and private herds in and around Jammu. Samples were detected for the presence of group A rotavirus using Latex agglutination test (LAT), Monoclonal Antibody based Enzyme Linked Immunosorbent Assay (mAb ELISA), RNA Polyacrylamide Gel Electrophoresis (RNA PAGE) and Reverse Transcription - Polymerase Chain Reaction (RT PCR). Samples positive by RT PCR were subjected to nested multiplex PCR for genotyping. 22.54% bovine faecal samples and 9.30% ovine faecal samples were tested positive for group A rotavirus by LAT. 14.08% bovine samples and 6.98% ovine samples were tested positive for group A rotavirus by ELISA. RNA PAGE yielded 12.68% bovine samples and 4.07% ovine samples as positive. 16.90% bovine samples and 4.65% ovine samples were positive by RT PCR. Samples positive by RTPCR were subjected to genotyping. 58.33% bovine rotavirus samples were typed as G6, 16.66% as G10 while 25% bovine rotavirus could not be typed. In case of ovine rotavirus 50% were genotyped as G6, 25% samples having mixed infection with G6 and G10, and 12.5% sample were typed as G10, while 12.5% were untypeable. For P typing 91.66% bovine rotavirus were typed as P[11], no sample was typed as P[5] while 8.33% could not be typed. Similarly in ovine, among RT PCR positive samples 62.5% samples were typed as P[11], no sample was typed as P[5] while 37.5% sample could not be typed using this set of primers.
