Pathobiological and molecular characterization of an Indian isolate of pigeon paramyxovirus type-I

The biological and molecular characterization of a pigeon paramyxovirus type-1 (PPMV-1) isolated from pigeon in Bareilly, India, showing typical symptoms of greenish diarrhoea and nervous signs like tremors of head, incoordination, torticollis and paralysis of legs and wings have been described. The MDT, ICPI and IVPI of the isolate were 112hr, 0.42 and 0.00, respectively, suggesting it to be lentogenic. However, pigeons inoculated with the isolate became sick and died. The fusion ‘F’ protein gene of the virus was partially amplified and sequenced. The amino acid sequence at the F1/F2 cleavage site was ¹¹² RRQKRF¹¹⁷typical of virulent NDV. These results provide further evidence about the unusual pathogenicity of PPMV-1 for chickens.