In vivo chemotactic evaluation of ferrous ion in piglets

An experiment was conducted to evaluate the chemotactic activity of free iron on piglet skin by different treatments of ferrous sulphate injection. It was found that free iron plays a major role as endogenous chemical mediator of inflammation. Intravenous injection of the same agent prior to induction of inflammation confirmed the role of gradient concentration in determining the direction of movement of leucocytes. Similarly, pretreatment of skin with EDTA and Amlodipine also caused a significant decrease in emigration of leucocytes from blood vessels at almost all periods of observation, confirming the essential requirement of calcium for cellular migration.